4QA8

Crystal structure of LprF from Mycobacterium bovis

4QA8 の概要

• エントリーDOI: 10.2210/pdb4qa8/pdb
• 分子名称: Putative lipoprotein LprF, (2R)-2-(dodecanoyloxy)propyl (4E,6E,8E,10E,12E)-pentadeca-4,6,8,10,12-pentaenoate (3 entities in total)
• 機能のキーワード: lipid transfer, diacylated glycolipid, lipid transport
• 由来する生物種: Mycobacterium bovis
• 細胞内の位置: Cell membrane ; Lipid-anchor : P65315
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24200.45

構造登録者

Ha, N.C.,Jiao, L.,Kim, J.S. (登録日: 2014-05-02, 公開日: 2014-10-22, 最終更新日: 2024-03-20)

主引用文献

Kim, J.S.,Jiao, L.,Oh, J.I.,Ha, N.C.,Kim, Y.H.
Crystal structure and functional implications of LprF from Mycobacterium tuberculosis and M. bovis
Acta Crystallogr.,Sect.D, 70:2619-2630, 2014

PubMed Abstract: The Gram-positive bacteria Mycobacterium tuberculosis and M. bovis are causative agents of tuberculosis in humans and cattle. The lipoprotein LprF is found in M. tuberculosis and M. bovis but not in the nonpathogenic M. smegmatis. To date, the role of LprF remains to be elucidated. In this study, the crystal structure of LprF has been determined at 1.1 Å resolution. The overall structure is similar to that of a homologue, LprG, with a central hydrophobic cavity that binds a triacylated glycolipid. LprF exhibited a central cavity structure similar to that of LprG, but with a smaller cavity that binds two alkyl chains. Consistently, subsequent mass-spectrometric analysis revealed that the bound ligand was a diacylated glycolipid, as found in the structure. Furthermore, an increased ratio of lipoarabinomannan to lipomannan in the mycobacterial cell wall was observed when lprF was introduced into M. smegmatis. These observations suggested that LprF transfers the diacylated glycolipid from the plasma membrane to the cell wall, which might be related to the pathogenesis of the bacteria.

PubMed: 25286846
DOI: 10.1107/S1399004714016599

実験手法

X-RAY DIFFRACTION (1.1 Å)