4Q7D

Wild type Fc (wtFc)

4Q7D の概要

• エントリーDOI: 10.2210/pdb4q7d/pdb
• 関連するPDBエントリー: 4Q6Y 4Q74
• 分子名称: Ig gamma-1 chain C region, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: immunoglobulin fold, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P01857
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52661.00

構造登録者

Ahmed, A.A.,Giddens, J.,Pincetic, A.,Lomino, J.V.,Ravetch, J.V.,Wang, L.X.,Bjorkman, P.J. (登録日: 2014-04-24, 公開日: 2014-07-23, 最終更新日: 2024-10-30)

主引用文献

Ahmed, A.A.,Giddens, J.,Pincetic, A.,Lomino, J.V.,Ravetch, J.V.,Wang, L.X.,Bjorkman, P.J.
Structural characterization of anti-inflammatory immunoglobulin g fc proteins.
J.Mol.Biol., 426:3166-3179, 2014

PubMed Abstract: Immunoglobulin G (IgG) is a central mediator of host defense due to its ability to recognize and eliminate pathogens. The recognition and effector responses are encoded on distinct regions of IgGs. The diversity of the antigen recognition Fab domains accounts for IgG's ability to bind with high specificity to essentially any antigen. Recent studies have indicated that the Fc effector domain also displays considerable heterogeneity, accounting for its complex effector functions of inflammation, modulation, and immune suppression. Therapeutic anti-tumor antibodies, for example, require the pro-inflammatory properties of the IgG Fc to eliminate tumor cells, while the anti-inflammatory activity of intravenous IgG requires specific Fc glycans for activity. In particular, the anti-inflammatory activity of intravenous IgG is ascribed to a small population of IgGs in which the Asn297-linked complex N-glycans attached to each Fc CH2 domain include terminal α2,6-linked sialic acids. We used chemoenzymatic glycoengineering to prepare fully disialylated IgG Fc and solved its crystal structure. Comparison of the structures of asialylated Fc, sialylated Fc, and F241A Fc, a mutant that displays increased glycan sialylation, suggests that increased conformational flexibility of the CH2 domain is associated with the switch from pro-inflammatory to anti-inflammatory activity of the Fc.

PubMed: 25036289
DOI: 10.1016/j.jmb.2014.07.006

実験手法

X-RAY DIFFRACTION (2.35 Å)