4Q56
Structure of Helix aspersa agglutinin with natural glycosylation and N-acetyl-alpha-D-galactosamine (GalNAc)
Summary for 4Q56
| Entry DOI | 10.2210/pdb4q56/pdb |
| Related | 2CCV 2CE6 2CGY 2CGZ |
| Descriptor | Helix aspersa agglutinin (HAA), alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-4)-alpha-L-fucopyranose-(1-3)-[beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose, ACETATE ION, ... (7 entities in total) |
| Functional Keywords | carbohydrate-binding protein, sugar binding protein, h-type lectin, snail mucus |
| Biological source | Helix aspersa |
| Total number of polymer chains | 1 |
| Total formula weight | 12755.38 |
| Authors | Pietrzyk, A.J.,Bujacz, A.,Bujacz, G. (deposition date: 2014-04-16, release date: 2015-10-14, Last modification date: 2023-09-20) |
| Primary citation | Pietrzyk, A.J.,Bujacz, A.,Mak, P.,Potempa, B.,Niedziela, T. Structural studies of Helix aspersa agglutinin complexed with GalNAc: A lectin that serves as a diagnostic tool. Int.J.Biol.Macromol., 81:1059-1068, 2015 Cited by PubMed Abstract: Lectins belong to a differentiated group of proteins known to possess sugar-binding properties. Due to this fact, they are interesting research targets in medical diagnostics. Helix aspersa agglutinin (HAA) is a lectin that recognizes the epitopes containing α-d-N-acetylgalactosamine (GalNAc), which is present at the surface of metastatic cancer cells. Although several reports have already described the use of HAA as a diagnostic tool, this protein was not characterized on the molecular level. Here, we present for the first time the structural information about lectin isolated from mucus of Helix aspersa (garden snail). The amino acid sequence of this agglutinin was determined by Edman degradation and tertiary as well as quaternary structure by X-ray crystallography. The high resolution crystal structure (1.38Å) and MALDI-TOF mass spectrometry analysis provide the detailed information about a large part of the HAA natural glycan chain. The topology of the GalNAc binding cleft and interaction with lectin are very well defined in the structure and fully confirmed by STD HSQC NMR spectroscopy. Together, this provides structural clues regarding HAA specificity and opens possibilities to rational modifications of this important diagnostic tool. PubMed: 26416237DOI: 10.1016/j.ijbiomac.2015.09.044 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.38 Å) |
Structure validation
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