4Q4Z

Thermus thermophilus RNA polymerase de novo transcription initiation complex

4Q4Z の概要

• エントリーDOI: 10.2210/pdb4q4z/pdb
• 関連するPDBエントリー: 4Q5A 4Q5S
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine, ... (12 entities in total)
• 機能のキーワード: transcription, dna and ntp, transcription-dna complex, transcription/dna
• 由来する生物種: Thermus thermophilus; 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q5SKW1
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 443004.47

構造登録者

Murakami, K.S. (登録日: 2014-04-15, 公開日: 2014-07-30, 最終更新日: 2024-02-28)

主引用文献

Basu, R.S.,Warner, B.A.,Molodtsov, V.,Pupov, D.,Esyunina, D.,Fernandez-Tornero, C.,Kulbachinskiy, A.,Murakami, K.S.
Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme.
J.Biol.Chem., 289:24549-24559, 2014

PubMed Abstract: The bacterial RNA polymerase (RNAP) holoenzyme containing σ factor initiates transcription at specific promoter sites by de novo RNA priming, the first step of RNA synthesis where RNAP accepts two initiating ribonucleoside triphosphates (iNTPs) and performs the first phosphodiester bond formation. We present the structure of de novo transcription initiation complex that reveals unique contacts of the iNTPs bound at the transcription start site with the template DNA and also with RNAP and demonstrate the importance of these contacts for transcription initiation. To get further insight into the mechanism of RNA priming, we determined the structure of initially transcribing complex of RNAP holoenzyme with 6-mer RNA, obtained by in crystallo transcription approach. The structure highlights RNAP-RNA contacts that stabilize the short RNA transcript in the active site and demonstrates that the RNA 5'-end displaces σ region 3.2 from its position near the active site, which likely plays a key role in σ ejection during the initiation-to-elongation transition. Given the structural conservation of the RNAP active site, the mechanism of de novo RNA priming appears to be conserved in all cellular RNAPs.

PubMed: 24973216
DOI: 10.1074/jbc.M114.584037

実験手法

X-RAY DIFFRACTION (2.9 Å)