4Q2L

Atomic Resolution Structure of the E. coli YajR Transporter YAM Domain

Summary for 4Q2L

• Entry DOI: 10.2210/pdb4q2l/pdb
• Related: 3DXS 4Q2M
• Descriptor: Major facilitator superfamily MFS_1, ACETIC ACID, CADMIUM ION, ... (4 entities in total)
• Functional Keywords: feredoxin fold, transporter, transport protein
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 8203.83

Authors

Zhang, X.C. (deposition date: 2014-04-09, release date: 2014-07-09, Last modification date: 2024-05-29)

Primary citation

Jiang, D.,Zhao, Y.,Fan, J.,Liu, X.,Wu, Y.,Feng, W.,Zhang, X.C.
Atomic resolution structure of the E. coli YajR transporter YAM domain.
Biochem.Biophys.Res.Commun., 450:929-935, 2014

PubMed Abstract: YajR is an Escherichia coli transporter that belongs to the major facilitator superfamily. Unlike most MFS transporters, YajR contains a carboxyl terminal, cytosolic domain of 67 amino acid residues termed YAM domain. Although it is speculated that the function of this small soluble domain is to regulate the conformational change of the 12-helix transmembrane domain, its precise regulatory role remains unclear. Here, we report the crystal structure of the YAM domain at 1.07-Å resolution, along with its structure determined using nuclear magnetic resonance. Detailed analysis of the high resolution structure revealed a symmetrical dimer in which a belt of well-ordered poly-pentagonal water molecules is embedded. A mutagenesis experiment and a thermal stability assay were used to analyze the putative role of this dimerization in response to changes in halogen concentration.

PubMed: 24952155
DOI: 10.1016/j.bbrc.2014.06.053

Experimental method

X-RAY DIFFRACTION (1.071 Å)