4PQZ

Crystal structure of swt1 C-terminal domain from yeast

4PQZ の概要

• エントリーDOI: 10.2210/pdb4pqz/pdb
• 分子名称: Transcriptional protein SWT1 (2 entities in total)
• 機能のキーワード: hepn like fold, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Nucleus : Q12104
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19645.99

構造登録者

Peng, S.X.,Gao, Z.Q.,Liu, Q.S.,Dong, Y.H. (登録日: 2014-03-05, 公開日: 2014-12-03, 最終更新日: 2024-11-06)

主引用文献

Peng, S.,Zhou, K.,Wang, W.,Gao, Z.,Dong, Y.,Liu, Q.
High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1.
Biochem.Biophys.Res.Commun., 453:826-832, 2014

PubMed Abstract: Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance.

PubMed: 25450355
DOI: 10.1016/j.bbrc.2014.10.040

実験手法

X-RAY DIFFRACTION (2.3 Å)