4PQV

Crystal structure of an Xrn1-resistant RNA from the 3' untranslated region of a flavivirus (Murray Valley Encephalitis virus)

4PQV の概要

• エントリーDOI: 10.2210/pdb4pqv/pdb
• 分子名称: XRN1-resistant flaviviral RNA, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: flavivirus, 3' untranslated region, sfrna, pseudoknot, type c rna three-way junction, xrn1 resistant rna, xrrna), resists degradation, exonuclease xrn1, small flaviviral rna, rna
• 由来する生物種: Murray Valley encephalitis virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22313.93

構造登録者

Chapman, E.G.,Costantino, D.A.,Rabe, J.L.,Moon, S.L.,Wilusz, J.,Nix, J.C.,Kieft, J.S. (登録日: 2014-03-04, 公開日: 2014-04-30, 最終更新日: 2024-02-28)

主引用文献

Chapman, E.G.,Costantino, D.A.,Rabe, J.L.,Moon, S.L.,Wilusz, J.,Nix, J.C.,Kieft, J.S.
The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production.
Science, 344:307-310, 2014

PubMed Abstract: Flaviviruses are emerging human pathogens and worldwide health threats. During infection, pathogenic subgenomic flaviviral RNAs (sfRNAs) are produced by resisting degradation by the 5'→3' host cell exonuclease Xrn1 through an unknown RNA structure-based mechanism. Here, we present the crystal structure of a complete Xrn1-resistant flaviviral RNA, which contains interwoven pseudoknots within a compact structure that depends on highly conserved nucleotides. The RNA's three-dimensional topology creates a ringlike conformation, with the 5' end of the resistant structure passing through the ring from one side of the fold to the other. Disruption of this structure prevents formation of sfRNA during flaviviral infection. Thus, sfRNA formation results from an RNA fold that interacts directly with Xrn1, presenting the enzyme with a structure that confounds its helicase activity.

PubMed: 24744377
DOI: 10.1126/science.1250897

実験手法

X-RAY DIFFRACTION (2.463 Å)