4PQI

Crystal structure of glutathione transferase lambda3 from Populus trichocarpa

4PQI の概要

• エントリーDOI: 10.2210/pdb4pqi/pdb
• 関連するPDBエントリー: 4PQH
• 分子名称: In2-1 family protein, glutathione transferase lambda3, GLUTATHIONE, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: gst fold, thiol-tansferase, transferase
• 由来する生物種: Populus trichocarpa (western balsam poplar)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27959.08

構造登録者

Lallement, P.A.,Meux, E.,Gualberto, J.M.,Prosper, P.,Didierjean, C.,Haouz, A.,Saul, F.,Rouhier, N.,Hecker, A. (登録日: 2014-03-03, 公開日: 2014-06-25, 最終更新日: 2023-09-20)

主引用文献

Lallement, P.A.,Meux, E.,Gualberto, J.M.,Prosper, P.,Didierjean, C.,Saul, F.,Haouz, A.,Rouhier, N.,Hecker, A.
Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants.
Biochem.J., 462:39-52, 2014

PubMed Abstract: GSTs represent a superfamily of multifunctional proteins which play crucial roles in detoxification processes and secondary metabolism. Instead of promoting the conjugation of glutathione to acceptor molecules as do most GSTs, members of the Lambda class (GSTLs) catalyse deglutathionylation reactions via a catalytic cysteine residue. Three GSTL genes (Pt-GSTL1, Pt-GSTL2 and Pt-GSTL3) are present in Populus trichocarpa, but two transcripts, differing in their 5' extremities, were identified for Pt-GSTL3. Transcripts for these genes were primarily found in flowers, fruits, petioles and buds, but not in leaves and roots, suggesting roles associated with secondary metabolism in these organs. The expression of GFP-fusion proteins in tobacco showed that Pt-GSTL1 is localized in plastids, whereas Pt-GSTL2 and Pt-GSTL3A and Pt-GSTL3B are found in both the cytoplasm and the nucleus. The resolution of Pt-GSTL1 and Pt-GSTL3 structures by X-ray crystallography indicated that, although these proteins adopt a canonical GST fold quite similar to that found in dimeric Omega GSTs, their non-plant counterparts, they are strictly monomeric. This might explain some differences in the enzymatic properties of both enzyme types. Finally, from competition experiments between aromatic substrates and a fluorescent probe, we determined that the recognition of glutathionylated substrates is favoured over non-glutathionylated forms.

PubMed: 24825169
DOI: 10.1042/BJ20140390

実験手法

X-RAY DIFFRACTION (1.95 Å)