4PNJ

Recombinant Sperm Whale P6 Myoglobin Solved with Single Pulse Free Electron Laser Data

4PNJ の概要

• エントリーDOI: 10.2210/pdb4pnj/pdb
• 分子名称: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: myoglobin, femtosecond x-ray crystallography, oxygen transport
• 由来する生物種: Physeter catodon (Sperm whale)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18751.14

構造登録者

Cohen, A.,Gonzalez, A.,Lam, W.,Lyubimov, A.,Sauter, N.,Tsai, Y.,Uervirojnangkoorn, M.,Brunger, A.,Soltis, M. (登録日: 2014-05-23, 公開日: 2014-11-05, 最終更新日: 2023-09-27)

主引用文献

Cohen, A.E.,Soltis, S.M.,Gonzalez, A.,Aguila, L.,Alonso-Mori, R.,Barnes, C.O.,Baxter, E.L.,Brehmer, W.,Brewster, A.S.,Brunger, A.T.,Calero, G.,Chang, J.F.,Chollet, M.,Ehrensberger, P.,Eriksson, T.L.,Feng, Y.,Hattne, J.,Hedman, B.,Hollenbeck, M.,Holton, J.M.,Keable, S.,Kobilka, B.K.,Kovaleva, E.G.,Kruse, A.C.,Lemke, H.T.,Lin, G.,Lyubimov, A.Y.,Manglik, A.,Mathews, I.I.,McPhillips, S.E.,Nelson, S.,Peters, J.W.,Sauter, N.K.,Smith, C.A.,Song, J.,Stevenson, H.P.,Tsai, Y.,Uervirojnangkoorn, M.,Vinetsky, V.,Wakatsuki, S.,Weis, W.I.,Zadvornyy, O.A.,Zeldin, O.B.,Zhu, D.,Hodgson, K.O.
Goniometer-based femtosecond crystallography with X-ray free electron lasers.
Proc.Natl.Acad.Sci.USA, 111:17122-17127, 2014

PubMed Abstract: The emerging method of femtosecond crystallography (FX) may extend the diffraction resolution accessible from small radiation-sensitive crystals and provides a means to determine catalytically accurate structures of acutely radiation-sensitive metalloenzymes. Automated goniometer-based instrumentation developed for use at the Linac Coherent Light Source enabled efficient and flexible FX experiments to be performed on a variety of sample types. In the case of rod-shaped Cpl hydrogenase crystals, only five crystals and about 30 min of beam time were used to obtain the 125 still diffraction patterns used to produce a 1.6-Å resolution electron density map. For smaller crystals, high-density grids were used to increase sample throughput; 930 myoglobin crystals mounted at random orientation inside 32 grids were exposed, demonstrating the utility of this approach. Screening results from cryocooled crystals of β2-adrenoreceptor and an RNA polymerase II complex indicate the potential to extend the diffraction resolution obtainable from very radiation-sensitive samples beyond that possible with undulator-based synchrotron sources.

PubMed: 25362050
DOI: 10.1073/pnas.1418733111

実験手法

X-RAY DIFFRACTION (1.36 Å)