4PL6

Structure of the chromodomain of MRG2 in complex with H3K4me3

4PL6 の概要

• エントリーDOI: 10.2210/pdb4pl6/pdb
• 関連するPDBエントリー: 4PLI 4PLL
• 分子名称: At1g02740, H3K4me3 (3 entities in total)
• 機能のキーワード: chromo domain, h3k4me3, transcription
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• 細胞内の位置: Nucleus : Q4V3E2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 20823.42

構造登録者

Liu, Y.C.,Huang, Y. (登録日: 2014-05-16, 公開日: 2015-07-22, 最終更新日: 2023-12-27)

主引用文献

Bu, Z.,Yu, Y.,Li, Z.,Liu, Y.,Jiang, W.,Huang, Y.,Dong, A.W.
Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Plos Genet., 10:e1004617-e1004617, 2014

PubMed Abstract: Day-length is important for regulating the transition to reproductive development (flowering) in plants. In the model plant Arabidopsis thaliana, the transcription factor CONSTANS (CO) promotes expression of the florigen FLOWERING LOCUS T (FT), constituting a key flowering pathway under long-day photoperiods. Recent studies have revealed that FT expression is regulated by changes of histone modification marks of the FT chromatin, but the epigenetic regulators that directly interact with the CO protein have not been identified. Here, we show that the Arabidopsis Morf Related Gene (MRG) group proteins MRG1 and MRG2 act as H3K4me3/H3K36me3 readers and physically interact with CO to activate FT expression. In vitro binding analyses indicated that the chromodomains of MRG1 and MRG2 preferentially bind H3K4me3/H3K36me3 peptides. The mrg1 mrg2 double mutant exhibits reduced mRNA levels of FT, but not of CO, and shows a late-flowering phenotype under the long-day but not short-day photoperiod growth conditions. MRG2 associates with the chromatin of FT promoter in a way dependent of both CO and H3K4me3/H3K36me3. Vice versa, loss of MRG1 and MRG2 also impairs CO binding at the FT promoter. Crystal structure analyses of MRG2 bound with H3K4me3/H3K36me3 peptides together with mutagenesis analysis in planta further demonstrated that MRG2 function relies on its H3K4me3/H3K36me3-binding activity. Collectively, our results unravel a novel chromatin regulatory mechanism, linking functions of MRG1 and MRG2 proteins, H3K4/H3K36 methylations, and CO in FT activation in the photoperiodic regulation of flowering time in plants.

PubMed: 25211338
DOI: 10.1371/journal.pgen.1004617

実験手法

X-RAY DIFFRACTION (1.681 Å)