4PKO

Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex

4PKO の概要

• エントリーDOI: 10.2210/pdb4pko/pdb
• 関連するPDBエントリー: 4PKN
• 分子名称: 60 kDa chaperonin, 10 kDa chaperonin, ADENOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: chaperonin, protein encapsulation, groel-groes2 complex, protein binding
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q548M1 Q7BGE6
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 956889.64

構造登録者

Fei, X.,Ye, X.,Laronde-Leblanc, N.,Lorimer, G.H. (登録日: 2014-05-15, 公開日: 2014-08-20, 最終更新日: 2024-10-09)

主引用文献

Fei, X.,Ye, X.,LaRonde, N.A.,Lorimer, G.H.
Formation and structures of GroEL:GroES2 chaperonin footballs, the protein-folding functional form.
Proc.Natl.Acad.Sci.USA, 111:12775-12780, 2014

PubMed Abstract: The GroE chaperonins assist substrate protein (SP) folding by cycling through several conformational states. With each cycle the SP is, in turn, captured, unfolded, briefly encapsulated (t1/2 ∼ 1 s), and released by the chaperonin complex. The protein-folding functional form is the US-football-shaped GroEL:GroES2 complex. We report structures of two such "football" complexes to ∼ 3.7-Å resolution; one is empty whereas the other contains encapsulated SP in both chambers. Although encapsulated SP is not visible on the electron density map, using calibrated FRET and order-of-addition experiments we show that owing to SP-catalyzed ADP/ATP exchange both chambers of the football complex encapsulate SP efficiently only if the binding of SP precedes that of ATP. The two rings of GroEL thus behave as a parallel processing machine, rather than functioning alternately. Compared with the bullet-shaped GroEL:GroES1 complex, the GroEL:GroES2 football complex differs conformationally at the GroEL-GroES interface and also at the interface between the two GroEL rings. We propose that the electrostatic interactions between the ε-NH(3+) of K105 of helix D in one ring with the negatively charged carboxyl oxygen of A109 at the carboxyl end of helix D of the other ring provide the structural basis for negative inter-ring cooperativity.

PubMed: 25136110
DOI: 10.1073/pnas.1412922111

実験手法

X-RAY DIFFRACTION (3.84 Å)