4PGO

Crystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data

4PGO の概要

• エントリーDOI: 10.2210/pdb4pgo/pdb
• 関連するPDBエントリー: 1XE1
• 分子名称: Uncharacterized protein, CHLORIDE ION (3 entities in total)
• 機能のキーワード: sulfur sad, southeast collaboratory for structural genomics, secsg, structural genomics, unknown function
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12774.74

構造登録者

Weinert, T.,Waltersperger, S.,Olieric, V.,Panepucci, E.,Chen, L.,Rose, J.P.,Wang, M.,Wang, B.C.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2014-05-02, 公開日: 2014-12-10, 最終更新日: 2023-12-27)

主引用文献

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination.
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

実験手法

X-RAY DIFFRACTION (2.3 Å)