4P35

Crystal structure of DJ-1 with Zinc(II) bound (crystal I)

4P35 の概要

• エントリーDOI: 10.2210/pdb4p35/pdb
• 関連するPDBエントリー: 4P2G 4P34 4P36
• 分子名称: Protein DJ-1, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: parkinson's disease, oxidation, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane ; Lipid-anchor : Q99497
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20110.01

構造登録者

Tashiro, S.,Wu, C.-X.,Hoang, Q.Q.,Caaveiro, J.M.M.,Tsumoto, K. (登録日: 2014-03-05, 公開日: 2014-04-09, 最終更新日: 2023-09-27)

主引用文献

Tashiro, S.,Caaveiro, J.M.,Wu, C.X.,Hoang, Q.Q.,Tsumoto, K.
Thermodynamic and Structural Characterization of the Specific Binding of Zn(II) to Human Protein DJ-1.
Biochemistry, 53:2218-2220, 2014

PubMed Abstract: Mutations of DJ-1 cause familial Parkinson's disease (PD), although the role of DJ-1 in PD remains unresolved. Very recent reports have shown that DJ-1 interacts with copper ions. This evidence opens new avenues to understanding the function of DJ-1 and its role in PD. Herein, we report that Zn(II) binds to DJ-1 with great selectivity among the other metals examined: Mn(II), Fe(II), Co(II), Ni(II), and Cu(II). High-resolution X-ray crystallography (1.18 Å resolution) shows Zn(II) is coordinated to the protein by the key residues Cys106 and Glu18. These results suggest that DJ-1 may be regulated and/or stabilized by Zn(II).

PubMed: 24697266
DOI: 10.1021/bi500294h

実験手法

X-RAY DIFFRACTION (1.75 Å)