4P1Q

GREEN FLUORESCENT PROTEIN E222H VARIANT

4P1Q の概要

• エントリーDOI: 10.2210/pdb4p1q/pdb
• 分子名称: Green fluorescent protein, SODIUM ION (3 entities in total)
• 機能のキーワード: chromophore, beta-barrel, luminescence, photoprotein, bioluminescence, fluorescent protein, luminescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25845.95

構造登録者

Klein, M.,Carius, Y.,Auerbach, D.,Franz, S.,Jung, G.,Lancaster, C.R.D. (登録日: 2014-02-27, 公開日: 2014-07-16, 最終更新日: 2024-11-06)

主引用文献

Auerbach, D.,Klein, M.,Franz, S.,Carius, Y.,Lancaster, C.R.,Jung, G.
Replacement of Highly Conserved E222 by the Photostable Non-photoconvertible Histidine in GFP.
Chembiochem, 15:1404-1408, 2014

PubMed Abstract: The widely used green fluorescent protein (GFP) decarboxylates upon irradiation; this involves removal of the acidic function of the glutamic acid at position 222, thereby resulting in the irreversible photoconversion of GFP. To suppress this phenomenon, the photostable, non-photoconvertible histidine was introduced at position 222 in GFP. The variant E222H shows negligible photodynamic processes and high expression yield. In addition, the stable and bright fluorescence over a wide pH range makes the E222H protein an alternative for GFP in fluorescence imaging and spectroscopy. Other fluorescent proteins are predicted to benefit from replacement of the catalytic glutamic acid by histidine.

PubMed: 24919579
DOI: 10.1002/cbic.201402075

実験手法

X-RAY DIFFRACTION (1.5 Å)