4OZS

RNA binding protein

4OZS の概要

• エントリーDOI: 10.2210/pdb4ozs/pdb
• 分子名称: Alpha solenoid protein (2 entities in total)
• 機能のキーワード: rna, specificity, rna binding protein
• 由来する生物種: artificial gene
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19480.52

構造登録者

Gully, B.S.,Bond, C.S. (登録日: 2014-02-19, 公開日: 2015-04-22, 最終更新日: 2024-10-30)

主引用文献

Gully, B.S.,Shah, K.R.,Lee, M.,Shearston, K.,Smith, N.M.,Sadowska, A.,Blythe, A.J.,Bernath-Levin, K.,Stanley, W.A.,Small, I.D.,Bond, C.S.
The design and structural characterization of a synthetic pentatricopeptide repeat protein.
Acta Crystallogr.,Sect.D, 71:196-208, 2015

PubMed Abstract: Proteins of the pentatricopeptide repeat (PPR) superfamily are characterized by tandem arrays of a degenerate 35-amino-acid α-hairpin motif. PPR proteins are typically single-stranded RNA-binding proteins with essential roles in organelle biogenesis, RNA editing and mRNA maturation. A modular, predictable code for sequence-specific binding of RNA by PPR proteins has recently been revealed, which opens the door to the de novo design of bespoke proteins with specific RNA targets, with widespread biotechnological potential. Here, the design and production of a synthetic PPR protein based on a consensus sequence and the determination of its crystal structure to 2.2 Å resolution are described. The crystal structure displays helical disorder, resulting in electron density representing an infinite superhelical PPR protein. A structural comparison with related tetratricopeptide repeat (TPR) proteins, and with native PPR proteins, reveals key roles for conserved residues in directing the structure and function of PPR proteins. The designed proteins have high solubility and thermal stability, and can form long tracts of PPR repeats. Thus, consensus-sequence synthetic PPR proteins could provide a suitable backbone for the design of bespoke RNA-binding proteins with the potential for high specificity.

PubMed: 25664731
DOI: 10.1107/S1399004714024869

実験手法

X-RAY DIFFRACTION (2.17 Å)