4OWC

PtI6 binding to HEWL

4OWC の概要

• エントリーDOI: 10.2210/pdb4owc/pdb
• 分子名称: Lysozyme C, IODIDE ION, PLATINUM (II) ION, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15901.35

構造登録者

Tanley, S.W.M.,Starkey, V.L.,Lamplough, L.,Kaenket, S.,Helliwell, J.R. (登録日: 2014-01-31, 公開日: 2014-09-24, 最終更新日: 2024-11-20)

主引用文献

Tanley, S.W.,Starkey, L.V.,Lamplough, L.,Kaenket, S.,Helliwell, J.R.
The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15.
Acta Crystallogr.,Sect.F, 70:1132-1134, 2014

PubMed Abstract: This study examines the binding and chemical stability of the platinum hexahalides K2PtCl6, K2PtBr6 and K2PtI6 when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI3 complex bound to the N(δ) atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI6) or at two sites (PtBr6 and PtCl6). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps as they would be recognisable 'objects'.

PubMed: 25195880
DOI: 10.1107/S2053230X14014009

実験手法

X-RAY DIFFRACTION (1.62 Å)