4OT2

Crystal Structure of Equine Serum Albumin in complex with Naproxen

4OT2 の概要

• エントリーDOI: 10.2210/pdb4ot2/pdb
• 関連するPDBエントリー: 4F5T 4F5U 4J2V
• 分子名称: Serum albumin, (2S)-2-(6-methoxynaphthalen-2-yl)propanoic acid, SUCCINIC ACID, ... (6 entities in total)
• 機能のキーワード: helical, three-domain protein, serum albumin superfamily, transport protein, fatty acids, metabolites and drugs, naproxen, plasma
• 由来する生物種: Equus caballus (domestic horse, equine)
• 細胞内の位置: Secreted: P35747
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66959.08

構造登録者

Sekula, B.,Bujacz, A.,Zielinski, K.,Bujacz, G. (登録日: 2014-02-13, 公開日: 2014-06-04, 最終更新日: 2024-11-20)

主引用文献

Bujacz, A.,Zielinski, K.,Sekula, B.
Structural studies of bovine, equine, and leporine serum albumin complexes with naproxen.
Proteins, 82:2199-2208, 2014

PubMed Abstract: Serum albumin, a protein naturally abundant in blood plasma, shows remarkable ligand binding properties of numerous endogenous and exogenous compounds. Most of serum albumin binding sites are able to interact with more than one class of ligands. Determining the protein-ligand interactions among mammalian serum albumins is essential for understanding the complexity of this transporter. We present three crystal structures of serum albumins in complexes with naproxen (NPS): bovine (BSA-NPS), equine (ESA-NPS), and leporine (LSA-NPS) determined to 2.58 Å (C2), 2.42 Å (P61), and 2.73 Å (P2₁2₁2₁) resolutions, respectively. A comparison of the structurally investigated complexes with the analogous complex of human serum albumin (HSA-NPS) revealed surprising differences in the number and distribution of naproxen binding sites. Bovine and leporine serum albumins possess three NPS binding sites, but ESA has only two. All three complexes of albumins studied here have two common naproxen locations, but BSA and LSA differ in the third NPS binding site. None of these binding sites coincides with the naproxen location in the HSA-NPS complex, which was obtained in the presence of other ligands besides naproxen. Even small differences in sequences of serum albumins from various species, especially in the area of the binding pockets, influence the affinity and the binding mode of naproxen to this transport protein.

PubMed: 24753230
DOI: 10.1002/prot.24583

実験手法

X-RAY DIFFRACTION (2.42 Å)