4OR1

Structure and mechanism of fibronectin binding and biofilm formation of enteroaggregative Escherischia coli AAF fimbriae

4OR1 の概要

• エントリーDOI: 10.2210/pdb4or1/pdb
• 分子名称: Invasin homolog AafB, Major fimbrial subunit of aggregative adherence fimbria II AafA chimeric construct, SULFATE ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: biofilm, chaperone-usher, pilus, adhesion, fibronectin, fimbriae, fibre, immunoglobulin-like, outer membrane pilus, cell adhesion
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34527.41

構造登録者

Lee, W.-C.,Garnett, J.A.,Yang, Y.,Matthews, S. (登録日: 2014-02-10, 公開日: 2014-12-24, 最終更新日: 2024-11-20)

主引用文献

Berry, A.A.,Yang, Y.,Pakharukova, N.,Garnett, J.A.,Lee, W.C.,Cota, E.,Marchant, J.,Roy, S.,Tuittila, M.,Liu, B.,Inman, K.G.,Ruiz-Perez, F.,Mandomando, I.,Nataro, J.P.,Zavialov, A.V.,Matthews, S.
Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.
Plos Pathog., 10:e1004404-e1004404, 2014

PubMed Abstract: Enteroaggregative Escherichia coli (EAEC) is a leading cause of acute and persistent diarrhea worldwide. A recently emerged Shiga-toxin-producing strain of EAEC resulted in significant mortality and morbidity due to progressive development of hemolytic-uremic syndrome. The attachment of EAEC to the human intestinal mucosa is mediated by aggregative adherence fimbria (AAF). Using X-ray crystallography and NMR structures, we present new atomic resolution insight into the structure of AAF variant I from the strain that caused the deadly outbreak in Germany in 2011, and AAF variant II from archetype strain 042, and propose a mechanism for AAF-mediated adhesion and biofilm formation. Our work shows that major subunits of AAF assemble into linear polymers by donor strand complementation where a single minor subunit is inserted at the tip of the polymer by accepting the donor strand from the terminal major subunit. Whereas the minor subunits of AAF have a distinct conserved structure, AAF major subunits display large structural differences, affecting the overall pilus architecture. These structures suggest a mechanism for AAF-mediated adhesion and biofilm formation. Binding experiments using wild type and mutant subunits (NMR and SPR) and bacteria (ELISA) revealed that despite the structural differences AAF recognize a common receptor, fibronectin, by employing clusters of basic residues at the junction between subunits in the pilus. We show that AAF-fibronectin attachment is based primarily on electrostatic interactions, a mechanism not reported previously for bacterial adhesion to biotic surfaces.

PubMed: 25232738
DOI: 10.1371/journal.ppat.1004404

実験手法

X-RAY DIFFRACTION (3 Å)