4OQ8

Satellite Tobacco Mosaic Virus Refined to 1.4 A Resolution using icosahedral constraints

Summary for 4OQ8

• Entry DOI: 10.2210/pdb4oq8/pdb
• Related: 1A34 4NIA 4OQ9
• Descriptor: Coat protein, RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3'), ... (8 entities in total)
• Functional Keywords: double-helix rna, protein-rna complex, swiss jelly-roll beta barrel, virus-rna complex, virus/rna
• Biological source: Satellite Tobacco Mosaic Virus (STMV); More
• Cellular location: Virion (Potential): P17574
• Total number of polymer chains: 4
• Total formula weight: 24676.53

Authors

Larson, S.B.,Day, J.S.,McPherson, A. (deposition date: 2014-02-07, release date: 2014-09-10, Last modification date: 2023-09-20)

Primary citation

Larson, S.B.,Day, J.S.,McPherson, A.
Satellite tobacco mosaic virus refined to 1.4 angstrom resolution.
Acta Crystallogr.,Sect.D, 70:2316-2330, 2014

PubMed Abstract: Satellite tobacco mosaic virus (STMV) is among the smallest viruses, having 60 identical subunits arranged with T = 1 icosahedral symmetry. Its crystal structure was solved at 290 K and was refined using, in part, crystals grown in microgravity. Electron-density maps revealed nearly 57% of the genomic ssRNA. Using six flash-cooled crystals, diffraction data were recorded to 1.4 Å resolution and independent refinements of the STMV model were carried out versus the previous 1.8 Å resolution data representing merged data from 21 crystals (271,689 unique reflections), data consisting of corresponding reflections to 1.8 Å resolution from the cooled crystals and 1.4 Å resolution data from the cooled crystals comprised of 570,721 unique reflections. Models were independently refined with full NCS constraints using the program CNS and in restrained mode using the programs CNS, REFMAC5 and SHELX-97, with the latter two procedures including anisotropic temperature factors. Significant additional structural detail emerged from the analyses, including a unique cation and anion arrangement on fivefold axes and a precise assessment of icosahedral symmetry exactness in the crystal lattice. STMV represents the highest resolution native virus structure currently known by a substantial margin, and it permits the evaluation of a precise atomic model of a spherical virus at near-atomic resolution for the first time.

PubMed: 25195746
DOI: 10.1107/S1399004714013789

Experimental method

X-RAY DIFFRACTION (1.45 Å)