4OP6

Urate OXIDASE + 8-AZAXANTHINE UNDER 40 BARS OXYGEN

4OP6 の概要

• エントリーDOI: 10.2210/pdb4op6/pdb
• 関連するPDBエントリー: 4op9
• 分子名称: Uricase, 8-AZAXANTHINE, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: homotetramer, t-fold domain, purine metabolism, oxidoreductase, oxygen binding, uric acid, peroxisome
• 由来する生物種: Aspergillus flavus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34391.68

構造登録者

Colloc'h, N.,Prange, T. (登録日: 2014-02-05, 公開日: 2014-03-19, 最終更新日: 2024-11-13)

主引用文献

Colloc'h, N.,Prange, T.
Functional relevance of the internal hydrophobic cavity of urate oxidase.
Febs Lett., 588:1715-1719, 2014

PubMed Abstract: Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavity leading to an inhibition of the catalytic activity. Analysis of the cavity volume variations and functional modifications suggest that the flexibility of the cavity would be an essential parameter for the active site efficiency. This cavity would act as a connecting vessel to give flexibility to the neighboring active site, and its expansion under pure oxygen pressure reveals that it might serve as a transient reservoir on its pathway to the active site.

PubMed: 24657440
DOI: 10.1016/j.febslet.2014.03.017

実験手法

X-RAY DIFFRACTION (1.65 Å)