4OOO

X-ray structure of the lysozyme derivative of tetrakis(acetato)chlorido diruthenium(II,III) complex

4OOO の概要

• エントリーDOI: 10.2210/pdb4ooo/pdb
• 関連するPDBエントリー: 4OOT
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15443.50

構造登録者

Merlino, A. (登録日: 2014-02-03, 公開日: 2014-06-18, 最終更新日: 2024-11-20)

主引用文献

Messori, L.,Marzo, T.,Sanches, R.N.,Hanif-Ur-Rehman,de Oliveira Silva, D.,Merlino, A.
Unusual Structural Features in the Lysozyme Derivative of the Tetrakis(acetato)chloridodiruthenium(II,III) Complex.
Angew.Chem.Int.Ed.Engl., 53:6172-6175, 2014

PubMed Abstract: The reaction between the paddle-wheel tetrakis(acetato)chloridodiruthenium(II,III) complex, [Ru2(μ-O2CCH3)4Cl] and hen egg-white lysozyme (HEWL) was investigated through ESI-MS and UV/Vis spectroscopy and the formation of a stable metal-protein adduct was unambiguously demonstrated. Remarkably, the diruthenium core is conserved in the adduct while two of the four acetate ligands are released. The crystal structure of this diruthenium-protein derivative was subsequently solved through X-ray diffraction analysis to 2.1 Å resolution. The structural data are in agreement with the solution results. It was found that HEWL binds two diruthenium moieties, at Asp101 and Asp119, respectively, with the concomitant release of two acetate ligands from each diruthenium center.

PubMed: 24796316
DOI: 10.1002/anie.201403337

実験手法

X-RAY DIFFRACTION (2.15 Å)