4OH4

Crystal structure of BRI1 in complex with BKI1

4OH4 の概要

• エントリーDOI: 10.2210/pdb4oh4/pdb
• 分子名称: Protein BRASSINOSTEROID INSENSITIVE 1, BRI1 kinase inhibitor 1, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: kinase domain, transferase, atp binding, phosphorylation, membrane, transferase-signaling protein complex, transferase/signaling protein
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: O22476; Cell membrane : Q9FMZ0
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80686.84

構造登録者

Wang, J.,Wang, J.,Wu, J.W.,Wang, Z.X. (登録日: 2014-01-17, 公開日: 2014-10-29, 最終更新日: 2024-11-20)

主引用文献

Wang, J.,Jiang, J.,Wang, J.,Chen, L.,Fan, S.L.,Wu, J.W.,Wang, X.,Wang, Z.X.
Structural insights into the negative regulation of BRI1 signaling by BRI1-interacting protein BKI1.
Cell Res., 24:1328-1341, 2014

PubMed Abstract: Brassinosteroids (BRs) are essential steroid hormones that have crucial roles in plant growth and development. BRs are perceived by the cell-surface receptor-like kinase brassinosteroid insensitive 1 (BRI1). In the absence of BRs, the cytosolic kinase domain (KD) of BRI1 is inhibited by its auto-inhibitory carboxyl terminus, as well as by interacting with an inhibitor protein, BRI1 kinase inhibitor 1 (BKI1). How BR binding to the extracellular domain of BRI1 leads to activation of the KD and dissociation of BKI1 into the cytosol remains unclear. Here we report the crystal structure of BRI1 KD in complex with the interacting peptide derived from BKI1. We also provide biochemical evidence that BRI1-associated kinase 1 (BAK1) plays an essential role in initiating BR signaling. Steroid-dependent heterodimerization of BRI1 and BAK1 ectodomains brings their cytoplasmic KDs in the right orientation for competing with BKI1 and transphosphorylation.

PubMed: 25331450
DOI: 10.1038/cr.2014.132

実験手法

X-RAY DIFFRACTION (2.25 Å)