4OD7

Complex structure of Proteus mirablis DsbA (C30S) with a non-covalently bound peptide PWATCDS

Summary for 4OD7

• Entry DOI: 10.2210/pdb4od7/pdb
• Related: 4OCE 4OCF
• Descriptor: Thiol:disulfide interchange protein, (ACE)PWATCDS(NH2) Peptide, THIOCYANATE ION, ... (4 entities in total)
• Functional Keywords: oxidative folding protein, virulence factor maturation protein, disulfide oxidoreductase, thioredoxin, dsba, dithiol exchange, dsbb, periplasmic, oxidoreductase-peptide complex, oxidoreductase/peptide
• Biological source: Proteus mirabilis
• Cellular location: Periplasm : B4EZ68
• Total number of polymer chains: 6
• Total formula weight: 65675.83

Authors

Kurth, F.,Premkumar, L.,Martin, J.L. (deposition date: 2014-01-10, release date: 2014-05-28, Last modification date: 2023-09-20)

Primary citation

Kurth, F.,Duprez, W.,Premkumar, L.,Schembri, M.A.,Fairlie, D.P.,Martin, J.L.
Crystal Structure of the Dithiol Oxidase DsbA Enzyme from Proteus Mirabilis Bound Non-covalently to an Active Site Peptide Ligand.
J.Biol.Chem., 289:19810-19822, 2014

PubMed Abstract: The disulfide bond forming DsbA enzymes and their DsbB interaction partners are attractive targets for development of antivirulence drugs because both are essential for virulence factor assembly in Gram-negative pathogens. Here we characterize PmDsbA from Proteus mirabilis, a bacterial pathogen increasingly associated with multidrug resistance. PmDsbA exhibits the characteristic properties of a DsbA, including an oxidizing potential, destabilizing disulfide, acidic active site cysteine, and dithiol oxidase catalytic activity. We evaluated a peptide, PWATCDS, derived from the partner protein DsbB and showed by thermal shift and isothermal titration calorimetry that it binds to PmDsbA. The crystal structures of PmDsbA, and the active site variant PmDsbAC30S were determined to high resolution. Analysis of these structures allows categorization of PmDsbA into the DsbA class exemplified by the archetypal Escherichia coli DsbA enzyme. We also present a crystal structure of PmDsbAC30S in complex with the peptide PWATCDS. The structure shows that the peptide binds non-covalently to the active site CXXC motif, the cis-Pro loop, and the hydrophobic groove adjacent to the active site of the enzyme. This high-resolution structural data provides a critical advance for future structure-based design of non-covalent peptidomimetic inhibitors. Such inhibitors would represent an entirely new antibacterial class that work by switching off the DSB virulence assembly machinery.

PubMed: 24831013
DOI: 10.1074/jbc.M114.552380

Experimental method

X-RAY DIFFRACTION (1.597 Å)