4O8B

Crystal structure of transcriptional regulator BswR

4O8B の概要

• エントリーDOI: 10.2210/pdb4o8b/pdb
• 分子名称: Uncharacterized protein (2 entities in total)
• 機能のキーワード: hth motif, transcriptional activator, promoter dna, dna binding protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14113.24

構造登録者

Ye, F.Z.,Wang, C.,Kumar, V.,Zhang, L.H.,Gao, Y.G. (登録日: 2013-12-26, 公開日: 2014-02-12, 最終更新日: 2023-11-08)

主引用文献

Wang, C.,Ye, F.,Kumar, V.,Gao, Y.G.,Zhang, L.H.
BswR controls bacterial motility and biofilm formation in Pseudomonas aeruginosa through modulation of the small RNA rsmZ.
Nucleic Acids Res., 42:4563-4576, 2014

PubMed Abstract: Pseudomonas aeruginosa relies on cell motility and ability to form biofilms to establish infections; however, the mechanism of regulation remains obscure. Here we report that BswR, a xenobiotic response element-type transcriptional regulator, plays a critical role in regulation of bacterial motility and biofilm formation in P. aeruginosa. Transcriptomic and biochemical analyses showed that BswR counteracts the repressor activity of MvaT, controls the transcription of small RNA rsmZ and regulates the biogenesis of bacterial flagella. The crystal structure of BswR was determined at 2.3 Å resolution; the monomer comprises a DNA-binding domain with a helix-turn-helix motif in the N terminus and two helices (α6 and α7) with a V-shaped arrangement in the C-terminus. In addition to the contacts between the parallel helices α5 of two monomers, the two helical extensions (α6 and α7) intertwine together to form a homodimer, which is the biological function unit. Based on the result of DNase I protection assay together with structural analysis of BswR homodimer, we proposed a BswR-DNA model, which suggests a molecular mechanism with which BswR could interact with DNA. Taken together, our results unveiled a novel regulatory mechanism, in which BswR controls the motility and biofilm formation of P. aeruginosa by modulating the transcription of small RNA rsmZ.

PubMed: 24497189
DOI: 10.1093/nar/gku106

実験手法

X-RAY DIFFRACTION (2.3 Å)