4O87
Crystal structure of a N-tagged Nuclease
Summary for 4O87
| Entry DOI | 10.2210/pdb4o87/pdb |
| Related | 4O88 |
| Descriptor | N-tagged Nuclease, CITRIC ACID, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | novel fold, nuclease, hydrolase |
| Biological source | Millerozyma acaciae |
| Total number of polymer chains | 2 |
| Total formula weight | 75597.60 |
| Authors | Chakravarty, A.K.,Smith, P.,Shuman, S. (deposition date: 2013-12-26, release date: 2014-10-15, Last modification date: 2024-02-28) |
| Primary citation | Chakravarty, A.K.,Smith, P.,Jalan, R.,Shuman, S. Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination. Cell Rep, 7:339-347, 2014 Cited by PubMed Abstract: tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT incises a synthetic tRNA(Gln(UUG)) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems. PubMed: 24726365DOI: 10.1016/j.celrep.2014.03.034 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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