4O2H

Crystal structure of BCAM1869 protein (RsaM homolog) from Burkholderia cenocepacia

4O2H の概要

• エントリーDOI: 10.2210/pdb4o2h/pdb
• 分子名称: protein BCAM1869 (2 entities in total)
• 機能のキーワード: structural genomics, psi-biology, midwest center for structural genomics, mcsg, quorum sensing, unknown function
• 由来する生物種: Burkholderia cenocepacia
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31336.30

構造登録者

Michalska, K.,Chhor, G.,Clancy, S.,Winans, S.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2013-12-17, 公開日: 2014-01-22, 最終更新日: 2024-11-20)

主引用文献

Michalska, K.,Chhor, G.,Clancy, S.,Jedrzejczak, R.,Babnigg, G.,Winans, S.C.,Joachimiak, A.
RsaM: a transcriptional regulator of Burkholderia spp. with novel fold.
Febs J., 281:4293-4306, 2014

PubMed Abstract: Burkholderia cepacia complex is a set of closely related bacterial species that are notorious pathogens of cystic fibrosis patients, responsible for life-threatening lung infections. Expression of several virulence factors of Burkholderia cepacia complex is controlled by a mechanism known as quorum sensing (QS). QS is a means of bacterial communication used to coordinate gene expression in a cell-density-dependent manner. The system involves the production of diffusible signaling molecules (N-acyl-l-homoserine lactones, AHLs), that bind to cognate transcriptional regulators and influence their ability to regulate gene expression. One such system that is highly conserved in Burkholderia cepacia complex consists of CepI and CepR. CepI is AHL synthase, whereas CepR is an AHL-dependent transcription factor. In most members of the Burkholderia cepacia complex group, the cepI and cepR genes are divergently transcribed and separated by additional genes. One of them, bcam1869, encodes the BcRsaM protein, which was recently postulated to modulate the abundance or activity of CepI or CepR. Here, we show the crystal structure of BcRsaM from B. cenocepacia J2315. It is a single-domain protein with unique topology and presents a novel fold. The protein is a dimer in the crystal and in solution. This regulator has no known DNA-binding motifs and direct binding of BcRsaM to the cepI promoter could not be detected in in vitro assays. Therefore, we propose that the modulatory action of RsaM might result from interactions with other components of the QS machinery rather than from direct association with the DNA promoter.

PubMed: 24916958
DOI: 10.1111/febs.12868

実験手法

X-RAY DIFFRACTION (2.3 Å)