4O2F

A peptide complexed with HLA-B*3901

4O2F の概要

• エントリーDOI: 10.2210/pdb4o2f/pdb
• 関連するPDBエントリー: 4O2C 4O2E
• 分子名称: HLA class I histocompatibility antigen, B-39 alpha chain, Beta-2-microglobulin, Peptide from ATP-dependent RNA helicase DDX3X, ... (4 entities in total)
• 機能のキーワード: ig-like, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P30475; Secreted: P61769; Nucleus speckle: O00571
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 88631.97

構造登録者

Sun, M.,Liu, J.,Qi, J.,Tefsen, B.,Shi, Y.,Yan, J.,Gao, G.F. (登録日: 2013-12-17, 公開日: 2014-07-23, 最終更新日: 2024-10-16)

主引用文献

Sun, M.,Liu, J.,Qi, J.,Tefsen, B.,Shi, Y.,Yan, J.,Gao, G.F.
N alpha-terminal acetylation for T cell recognition: molecular basis of MHC class I-restricted n alpha-acetylpeptide presentation
J.Immunol., 192:5509-5519, 2014

PubMed Abstract: As one of the most common posttranslational modifications (PTMs) of eukaryotic proteins, N(α)-terminal acetylation (Nt-acetylation) generates a class of N(α)-acetylpeptides that are known to be presented by MHC class I at the cell surface. Although such PTM plays a pivotal role in adjusting proteolysis, the molecular basis for the presentation and T cell recognition of N(α)-acetylpeptides remains largely unknown. In this study, we determined a high-resolution crystallographic structure of HLA (HLA)-B*3901 complexed with an N(α)-acetylpeptide derived from natural cellular processing, also in comparison with the unmodified-peptide complex. Unlike the α-amino-free P1 residues of unmodified peptide, of which the α-amino group inserts into pocket A of the Ag-binding groove, the N(α)-linked acetyl of the acetylated P1-Ser protrudes out of the groove for T cell recognition. Moreover, the Nt-acetylation not only alters the conformation of the peptide but also switches the residues in the α1-helix of HLA-B*3901, which may impact the T cell engagement. The thermostability measurements of complexes between N(α)-acetylpeptides and a series of MHC class I molecules derived from different species reveal reduced stability. Our findings provide the insight into the mode of N(α)-acetylpeptide-specific presentation by classical MHC class I molecules and shed light on the potential of acetylepitope-based immune intervene and vaccine development.

PubMed: 24829406
DOI: 10.4049/jimmunol.1400199

実験手法

X-RAY DIFFRACTION (1.901 Å)