4O1J

Crystal structures of two tetrameric beta-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora.

4O1J の概要

• エントリーDOI: 10.2210/pdb4o1j/pdb
• 関連するPDBエントリー: 4O1K
• 分子名称: Carbonic anhydrase, ZINC ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: carbon dioxide, inhibition, lyase
• 由来する生物種: Sordaria macrospora
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54994.42

構造登録者

Lehneck, R.,Neumann, P.,Vullo, D.,Elleuche, S.,Supuran, C.T.,Ficner, R.,Poggeler, S. (登録日: 2013-12-16, 公開日: 2014-03-05, 最終更新日: 2023-09-20)

主引用文献

Lehneck, R.,Neumann, P.,Vullo, D.,Elleuche, S.,Supuran, C.T.,Ficner, R.,Poggeler, S.
Crystal structures of two tetrameric beta-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora.
Febs J., 281:1759-1772, 2014

PubMed Abstract: Carbonic anhydrases (CAs) are metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate (hydrogen carbonate) and protons. CAs have been identified in archaea, bacteria and eukaryotes and can be classified into five groups (α, β, γ, δ, ζ) that are unrelated in sequence and structure. The fungal β-class has only recently attracted attention. In the present study, we investigated the structure and function of the plant-like β-CA proteins CAS1 and CAS2 from the filamentous ascomycete Sordaria macrospora. We demonstrated that both proteins can substitute for the Saccharomyces cerevisiae β-CA Nce103 and exhibit an in vitro CO2 hydration activity (kcat /Km of CAS1: 1.30 × 10(6) m(-1) ·s(-1) ; CAS2: 1.21 × 10(6 ) m(-1) ·s(-1) ). To further investigate the structural properties of CAS1 and CAS2, we determined their crystal structures to a resolution of 2.7 Å and 1.8 Å, respectively. The oligomeric state of both proteins is tetrameric. With the exception of the active site composition, no further major differences have been found. In both enzymes, the Zn(2) (+) -ion is tetrahedrally coordinated; in CAS1 by Cys45, His101 and Cys104 and a water molecule and in CAS2 by the side chains of four residues (Cys56, His112, Cys115 and Asp58). Both CAs are only weakly inhibited by anions, making them good candidates for industrial applications.

PubMed: 24506675
DOI: 10.1111/febs.12738

実験手法

X-RAY DIFFRACTION (2.695 Å)