4NT4

Crystal structure of the kinase domain of Gilgamesh isoform I from Drosophila melanogaster

4NT4 の概要

• エントリーDOI: 10.2210/pdb4nt4/pdb
• 分子名称: Gilgamesh, isoform I, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: serine/threonine protein kinase, wnt signaling pathway, transferase
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36706.27

構造登録者

Chen, C.C.,Shi, Z.B.,Zhou, Z.C. (登録日: 2013-11-30, 公開日: 2014-04-09, 最終更新日: 2023-11-08)

主引用文献

Han, N.,Chen, C.,Shi, Z.,Cheng, D.
Structure of the kinase domain of Gilgamesh from Drosophila melanogaster
Acta Crystallogr.,Sect.F, 70:438-443, 2014

PubMed Abstract: The CK1 family kinases regulate multiple cellular aspects and play important roles in Wnt/Wingless and Hedgehog signalling. The kinase domain of Drosophila Gilgamesh isoform I (Gilgamesh-I), a homologue of human CK1-γ, was purified and crystallized. Crystals of methylated Gilgamesh-I kinase domain with a D210A mutation diffracted to 2.85 Å resolution and belonged to space group P43212, with unit-cell parameters a = b = 52.025, c = 291.727 Å. The structure of Gilgamesh-I kinase domain, which was determined by molecular replacement, has conserved catalytic elements and an active conformation. Structural comparison indicates that an extended loop between the α1 helix and the β4 strand exists in the Gilgamesh-I kinase domain. This extended loop may regulate the activity and function of Gilgamesh-I.

PubMed: 24699734
DOI: 10.1107/S2053230X14004774

実験手法

X-RAY DIFFRACTION (2.86 Å)