4NNP

Crystal Structure of Apo Manganese ABC transporter MntC from Staphylococcus aureus bound to an antagonistic fab fragment

Summary for 4NNP

• Entry DOI: 10.2210/pdb4nnp/pdb
• Related: 1FVE 4K3V 4NNO
• Descriptor: Lipoprotein, Heavy chain of antagonistic fab fragment, Light chain of antagonistic fab fragment, ... (4 entities in total)
• Functional Keywords: abc superfamily atp binding cassette transporter, mntc, fab, apo, mrsa, transport protein-immune system complex, transport protein/immune system
• Biological source: Staphylococcus aureus subsp. aureus; More
• Total number of polymer chains: 6
• Total formula weight: 163644.69

Authors

Rouge, L.,Sudhamsu, J. (deposition date: 2013-11-18, release date: 2014-12-10, Last modification date: 2024-11-20)

Primary citation

Ahuja, S.,Rouge, L.,Swem, D.L.,Sudhamsu, J.,Wu, P.,Russell, S.J.,Alexander, M.K.,Tam, C.,Nishiyama, M.,Starovasnik, M.A.,Koth, C.M.
Structural analysis of bacterial ABC transporter inhibition by an antibody fragment.
Structure, 23:713-723, 2015

PubMed Abstract: Bacterial ATP-binding cassette (ABC) importers play critical roles in nutrient acquisition and are potential antibacterial targets. However, structural bases for their inhibition are poorly defined. These pathways typically rely on substrate binding proteins (SBPs), which are essential for substrate recognition, delivery, and transporter function. We report the crystal structure of a Staphylococcus aureus SBP for Mn(II), termed MntC, in complex with FabC1, a potent antibody inhibitor of the MntABC pathway. This pathway is essential and highly expressed during S. aureus infection and facilitates the import of Mn(II), a critical cofactor for enzymes that detoxify reactive oxygen species (ROS). Structure-based functional studies indicate that FabC1 sterically blocks a structurally conserved surface of MntC, preventing its interaction with the MntB membrane importer and increasing wild-type S. aureus sensitivity to oxidative stress by more than 10-fold. The results define an SBP blocking mechanism as the basis for ABC importer inhibition by an engineered antibody fragment.

PubMed: 25752540
DOI: 10.1016/j.str.2015.01.020

Experimental method

X-RAY DIFFRACTION (2.69 Å)