4NNP

Crystal Structure of Apo Manganese ABC transporter MntC from Staphylococcus aureus bound to an antagonistic fab fragment

4NNP の概要

• エントリーDOI: 10.2210/pdb4nnp/pdb
• 関連するPDBエントリー: 1FVE 4K3V 4NNO
• 分子名称: Lipoprotein, Heavy chain of antagonistic fab fragment, Light chain of antagonistic fab fragment, ... (4 entities in total)
• 機能のキーワード: abc superfamily atp binding cassette transporter, mntc, fab, apo, mrsa, transport protein-immune system complex, transport protein/immune system
• 由来する生物種: Staphylococcus aureus subsp. aureus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 163644.69

構造登録者

Rouge, L.,Sudhamsu, J. (登録日: 2013-11-18, 公開日: 2014-12-10, 最終更新日: 2024-11-20)

主引用文献

Ahuja, S.,Rouge, L.,Swem, D.L.,Sudhamsu, J.,Wu, P.,Russell, S.J.,Alexander, M.K.,Tam, C.,Nishiyama, M.,Starovasnik, M.A.,Koth, C.M.
Structural analysis of bacterial ABC transporter inhibition by an antibody fragment.
Structure, 23:713-723, 2015

PubMed Abstract: Bacterial ATP-binding cassette (ABC) importers play critical roles in nutrient acquisition and are potential antibacterial targets. However, structural bases for their inhibition are poorly defined. These pathways typically rely on substrate binding proteins (SBPs), which are essential for substrate recognition, delivery, and transporter function. We report the crystal structure of a Staphylococcus aureus SBP for Mn(II), termed MntC, in complex with FabC1, a potent antibody inhibitor of the MntABC pathway. This pathway is essential and highly expressed during S. aureus infection and facilitates the import of Mn(II), a critical cofactor for enzymes that detoxify reactive oxygen species (ROS). Structure-based functional studies indicate that FabC1 sterically blocks a structurally conserved surface of MntC, preventing its interaction with the MntB membrane importer and increasing wild-type S. aureus sensitivity to oxidative stress by more than 10-fold. The results define an SBP blocking mechanism as the basis for ABC importer inhibition by an engineered antibody fragment.

PubMed: 25752540
DOI: 10.1016/j.str.2015.01.020

実験手法

X-RAY DIFFRACTION (2.69 Å)