4NN9

REFINED ATOMIC STRUCTURES OF N9 SUBTYPE INFLUENZA VIRUS NEURAMINIDASE AND ESCAPE MUTANTS

4NN9 の概要

• エントリーDOI: 10.2210/pdb4nn9/pdb
• 分子名称: NEURAMINIDASE N9, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: hydrolase(o-glycosyl)
• 由来する生物種: Influenza A virus
• 細胞内の位置: Virion membrane (By similarity): P03472
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45485.41

構造登録者

Tulip, W.R.,Varghese, J.N.,Baker, A.T.,Vandonkelaar, A.,Laver, W.G.,Webster, R.G.,Colman, P.M. (登録日: 1991-03-28, 公開日: 1992-07-15, 最終更新日: 2024-10-09)

主引用文献

Tulip, W.R.,Varghese, J.N.,Baker, A.T.,van Donkelaar, A.,Laver, W.G.,Webster, R.G.,Colman, P.M.
Refined atomic structures of N9 subtype influenza virus neuraminidase and escape mutants.
J.Mol.Biol., 221:487-497, 1991

PubMed Abstract: The crystal structure of the N9 subtype neuraminidase of influenza virus was refined by simulated annealing and conventional techniques to an R-factor of 0.172 for data in the resolution range 6.0 to 2.2 A. The r.m.s. deviation from ideal values of bond lengths is 0.014 A. The structure is similar to that of N2 subtype neuraminidase both in secondary structure elements and in their connections. The three-dimensional structures of several escape mutants of neuraminidase, selected with antineuraminidase monoclonal antibodies, are also reported. In every case, structural changes associated with the point mutation are confined to the mutation site or to residues that are spatially immediately adjacent to it. The failure of antisera to cross-react between N2 and N9 subtypes may be correlated with the absence of conserved, contiguous surface structures of area 700 A2 or more.

PubMed: 1920429
DOI: 10.1016/0022-2836(91)80069-7

実験手法

X-RAY DIFFRACTION (2.3 Å)