4NKB

Crystal Structure of the cryptic polo box (CPB)of ZYG-1

4NKB の概要

• エントリーDOI: 10.2210/pdb4nkb/pdb
• 関連するPDBエントリー: 4G7N 4NK7
• 分子名称: Probable serine/threonine-protein kinase zyg-1, MAGNESIUM ION, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, ... (5 entities in total)
• 機能のキーワード: cryptic polo box, centriole biogenesis, centrosomes, transferase
• 由来する生物種: Caenorhabditis elegans (nematode)
• 細胞内の位置: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome: Q9GT24
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52982.21

構造登録者

Shimanovskaya, E.,Dong, G. (登録日: 2013-11-12, 公開日: 2014-08-27, 最終更新日: 2024-02-28)

主引用文献

Shimanovskaya, E.,Viscardi, V.,Lesigang, J.,Lettman, M.M.,Qiao, R.,Svergun, D.I.,Round, A.,Oegema, K.,Dong, G.
Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases.
Structure, 22:1090-1104, 2014

PubMed Abstract: Plk4 family kinases control centriole assembly. Plk4s target mother centrioles through an interaction between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C. elegans ZYG-1, which forms a Z-shaped dimer containing an intermolecular β sheet with an extended basic surface patch. Biochemical and in vivo analysis revealed that electrostatic interactions dock the ZYG-1 CPB basic patch onto the SPD-2-derived acidic region to promote ZYG-1 targeting and new centriole assembly. Analysis of a different crystal form of the Drosophila Plk4 (DmPlk4) CPB suggests that it also forms a Z-shaped dimer. Comparison of the ZYG-1 and DmPlk4 CPBs revealed structural changes in the ZYG-1 CPB that confer selectivity for binding SPD-2 over Asterless-derived acidic regions. Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly.

PubMed: 24980795
DOI: 10.1016/j.str.2014.05.009

実験手法

X-RAY DIFFRACTION (2.3 Å)