4N8X

The structure of Nostoc sp. PCC 7120 CcmL

4N8X の概要

• エントリーDOI: 10.2210/pdb4n8x/pdb
• 関連するPDBエントリー: 4N8F
• 分子名称: Carbon dioxide concentrating mechanism protein, SULFATE ION (3 entities in total)
• 機能のキーワード: ccml, stranded beta barrel, bacterial microcompartment vertex protein, carboxysome, structural protein
• 由来する生物種: Nostoc sp.
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 368403.78

構造登録者

Keeling, T.J.,Kimber, M.S. (登録日: 2013-10-18, 公開日: 2014-03-12, 最終更新日: 2023-09-20)

主引用文献

Keeling, T.J.,Samborska, B.,Demers, R.W.,Kimber, M.S.
Interactions and structural variability of beta-carboxysomal shell protein CcmL.
Photosynth.Res., 121:125-133, 2014

PubMed Abstract: CcmL is a small, pentameric protein that is argued to fill the vertices of β-carboxysomal shell. Here we report the structures of two CcmL orthologs, those from Nostoc sp. PCC 7120 and Thermosynechococcus elongatus BP-1. These structures broadly resemble those previously reported for other strains. However, the Nostoc CcmL structure shows an interesting pattern of behavior where two loops that map to the base of the pentamer adopt either an out or in conformation, with a consistent (over six pentamers) out-in-out-in-in pattern of protomers. The pentamers in this structure are also consistently organized into a back-to-back decamer, though evidence suggests that this is likely not present in solution. Förster resonance energy transfer experiments were able to show a weak interaction between CcmL and CcmK2 when CcmK2 was present at >100 μM. Since CcmK2 forms defined bodies with approximately 200 nm diameter at this concentration, this would support the idea that CcmL can only interact with CcmK2 at rare defect points in the growing shell.

PubMed: 24504539
DOI: 10.1007/s11120-014-9973-z

実験手法

X-RAY DIFFRACTION (1.93 Å)