4N8V

Crystal structure of killer cell immunoglobulin-like receptor KIR2DS2 in complex with HLA-A

4N8V の概要

• エントリーDOI: 10.2210/pdb4n8v/pdb
• 分子名称: Killer cell immunoglobulin-like receptor 2DS2, HLA class I histocompatibility antigen, A-11 alpha chain, Beta-2-microglobulin, ... (5 entities in total)
• 機能のキーワード: antigen presentation, cell lysing, kir binding, membrane, kir2ds2, activating kir, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P43631; Secreted: P13746; Virion membrane; Multi-pass membrane protein (Potential): P61769; Cell membrane; Single-pass type I membrane protein: Q76ZQ3
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 133829.00

構造登録者

Liu, J.X.,Ren, E.C. (登録日: 2013-10-18, 公開日: 2014-02-05, 最終更新日: 2024-11-06)

主引用文献

Liu, J.X.,Xiao, Z.W.,Ko, H.L.,Shen, M.X.,Ren, E.C.
Activating killer cell immunoglobulin-like receptor 2DS2 binds to HLA-A*11
Proc.Natl.Acad.Sci.USA, 111:2662-2667, 2014

PubMed Abstract: Inhibitory killer cell Ig-like receptors (KIRs) are known to recognize HLA ligands mainly of the HLA-C and Bw4 groups, but the ligands for KIRs are poorly understood. We report here the identification of the cognate ligand for the activating KIR 2DS2 as HLA-A*11:01. The crystal structure of the KIR2DS2-HLA-A*11:01 complex was solved at 2.5-Å resolution and revealed residue-binding characteristics distinct from those of inhibitory KIRs with HLA-C and the critical role of residues Tyr45 and Asp72 in shaping binding specificity to HLA-A*11:01. Using KIR2DS2 tetramers, binding to surface HLA-A*11:01 on live cells was demonstrated and, furthermore, that binding can be altered by residue changes at p8 of the peptide, indicating the influence of peptide sequence on KIR-HLA association. In addition, heteronuclear single quantum coherence NMR was used to map the involvement of critical residues in HLA binding at the interface of KIR and HLA, and validates the data observed in the crystal structure. Our data provide structural evidence of the recognition of A*11:01 by the activating KIR2DS2 and extend our understanding of the KIR-HLA binding spectrum.

PubMed: 24550293
DOI: 10.1073/pnas.1322052111

実験手法

X-RAY DIFFRACTION (2.5 Å)