4N59

The Crystal Structure of Pectocin M2 at 2.3 Angstroms

4N59 の概要

• エントリーDOI: 10.2210/pdb4n59/pdb
• 関連するPDBエントリー: 2XMX 4FZL 4FZM 4FZN 4G75 4N58
• 分子名称: Pectocin M2, FE2/S2 (INORGANIC) CLUSTER, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: lipid-ii hydrolase, bacteriocin, protein antibiotic, hydrolase
• 由来する生物種: Pectobacterium carotovorum subsp. brasiliensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62674.00

構造登録者

Zeth, K.,Grinter, R.,Roszak, A.W.,Cogdell, R.J.,Walker, D. (登録日: 2013-10-09, 公開日: 2014-06-04, 最終更新日: 2023-09-20)

主引用文献

Grinter, R.,Josts, I.,Zeth, K.,Roszak, A.W.,McCaughey, L.C.,Cogdell, R.J.,Milner, J.J.,Kelly, S.M.,Byron, O.,Walker, D.
Structure of the atypical bacteriocin pectocin M2 implies a novel mechanism of protein uptake.
Mol.Microbiol., 93:234-246, 2014

PubMed Abstract: The colicin-like bacteriocins are potent protein antibiotics that have evolved to efficiently cross the outer membrane of Gram-negative bacteria by parasitizing nutrient uptake systems. We have structurally characterized the colicin M-like bacteriocin, pectocin M2, which is active against strains of Pectobacterium spp. This unusual bacteriocin lacks the intrinsically unstructured translocation domain that usually mediates translocation of these bacteriocins across the outer membrane, containing only a single globular ferredoxin domain connected to its cytotoxic domain by a flexible α-helix, which allows it to adopt two distinct conformations in solution. The ferredoxin domain of pectocin M2 is homologous to plant ferredoxins and allows pectocin M2 to parasitize a system utilized by Pectobacterium to obtain iron during infection of plants. Furthermore, we identify a novel ferredoxin-containing bacteriocin pectocin P, which possesses a cytotoxic domain homologous to lysozyme, illustrating that the ferredoxin domain acts as a generic delivery module for cytotoxic domains in Pectobacterium.

PubMed: 24865810
DOI: 10.1111/mmi.12655

実験手法

X-RAY DIFFRACTION (2.3 Å)