4N2S

Crystal Structure of THA8 in complex with Zm1a-6 RNA

4N2S の概要

• エントリーDOI: 10.2210/pdb4n2s/pdb
• 関連するPDBエントリー: 4ME2 4N2Q
• 分子名称: THA8 RNA binding protein, Zm1a-6 RNA (3 entities in total)
• 機能のキーワード: pentatricopeptide repeat (ppr) protein, small intron rna binding, group ii intron rna splicing, chloroplast, splicing-rna complex, splicing/rna
• 由来する生物種: Brachypodium distachyon (Purple false brome); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31590.06

構造登録者

Ke, J.,Chen, R.Z.,Ban, T.,Zhou, X.E.,Gu, X.,Brunzelle, J.S.,Zhu, J.K.,Melcher, K.,Xu, H.E. (登録日: 2013-10-06, 公開日: 2013-10-30, 最終更新日: 2024-04-03)

主引用文献

Ke, J.,Chen, R.Z.,Ban, T.,Zhou, X.E.,Gu, X.,Tan, M.H.,Chen, C.,Kang, Y.,Brunzelle, J.S.,Zhu, J.K.,Melcher, K.,Xu, H.E.
Structural basis for RNA recognition by a dimeric PPR-protein complex.
Nat.Struct.Mol.Biol., 20:1377-1382, 2013

PubMed Abstract: Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Brachypodium distachyon THA8 either free of RNA or bound to two of the identified RNA sites. The apostructure reveals a THA8 monomer with five tandem PPR repeats arranged in a planar fold. The complexes of THA8 bound to the two short RNA fragments surprisingly reveal asymmetric THA8 dimers with the bound RNAs at the dimeric interface. RNA binding induces THA8 dimerization, with a conserved G nucleotide of the bound RNAs making extensive contacts with both monomers. Together, these results establish a new model of RNA recognition by RNA-induced formation of an asymmetric dimer of a PPR protein.

PubMed: 24186060
DOI: 10.1038/nsmb.2710

実験手法

X-RAY DIFFRACTION (3 Å)