4MZR

Crystal structure of a polypeptide p53 mutant bound to DNA

4MZR の概要

• エントリーDOI: 10.2210/pdb4mzr/pdb
• 関連するPDBエントリー: 4MZI
• 分子名称: Cellular tumor antigen p53, consensus DNA sense strand, consensus DNA anti-sense strand, ... (5 entities in total)
• 機能のキーワード: p53, tumor suppressor, protein-dna complex, dna binding, multidomain, oligomerization, antitumor protein/dna, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 124340.09

構造登録者

Emamzadah, S.T.,Tropia, L.,Vincenti, I.,Falquet, B.,Halazonetis, T.D. (登録日: 2013-09-30, 公開日: 2014-01-15, 最終更新日: 2023-09-20)

主引用文献

Emamzadah, S.,Tropia, L.,Vincenti, I.,Falquet, B.,Halazonetis, T.D.
Reversal of the DNA-Binding-Induced Loop L1 Conformational Switch in an Engineered Human p53 Protein.
J.Mol.Biol., 426:936-944, 2014

PubMed Abstract: The gene encoding the p53 tumor suppressor protein, a sequence-specific DNA binding transcription factor, is the most frequently mutated gene in human cancer. Crystal structures of homo-oligomerizing p53 polypeptides with specific DNA suggest that DNA binding is associated with a conformational switch. Specifically, in the absence of DNA, loop L1 of the p53 DNA binding domain adopts an extended conformation, whereas two p53 subunits switch to a recessed loop L1 conformation when bound to DNA as a tetramer. We previously designed a p53 protein, p53FG, with amino substitutions S121F and V122G targeting loop L1. These two substitutions enhanced the affinity of p53 for specific DNA yet, counterintuitively, decreased the residency time of p53 on DNA. Here, we confirmed these DNA binding properties of p53FG using a different method. We also determined by crystallography the structure of p53FG in its free state and bound to DNA as a tetramer. In the free state, loop L1 adopted a recessed conformation, whereas upon DNA binding, two subunits switched to the extended loop L1 conformation, resulting in a final structure that was very similar to that of wild-type p53 bound to DNA. Thus, altering the apo structure of p53 changed its DNA binding properties, even though the DNA-bound structure was not altered.

PubMed: 24374182
DOI: 10.1016/j.jmb.2013.12.020

実験手法

X-RAY DIFFRACTION (2.9 Å)