4MT5

Crystal structure of Mub-RV

4MT5 の概要

• エントリーDOI: 10.2210/pdb4mt5/pdb
• 関連するPDBエントリー: 3I57
• 分子名称: Mucus binding proteinn (2 entities in total)
• 機能のキーワード: ubiquitin-like beta-grasp fold, mucin binding, cell-surface, protein binding
• 由来する生物種: Lactobacillus reuteri
• 細胞内の位置: Secreted, cell wall ; Peptidoglycan-anchor : F8KCE4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40624.07

構造登録者

Etzold, S.,Juge, N.,Hemmings, A.M. (登録日: 2013-09-19, 公開日: 2014-08-06, 最終更新日: 2024-02-28)

主引用文献

Etzold, S.,Kober, O.I.,Mackenzie, D.A.,Tailford, L.E.,Gunning, A.P.,Walshaw, J.,Hemmings, A.M.,Juge, N.
Structural basis for adaptation of lactobacilli to gastrointestinal mucus.
ENVIRON.MICROBIOL., 16:888-903, 2014

PubMed Abstract: The mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the underpinning mechanisms of these interactions are not understood. Here, we provide structural and functional insights into the canonical mucus-binding protein (MUB), a multi-repeat cell-surface adhesin found in Lactobacillus inhabitants of the GI tract. X-ray crystallography together with small-angle X-ray scattering demonstrated a 'beads on a string' arrangement of repeats, generating 174 nm long protein fibrils, as shown by atomic force microscopy. Each repeat consists of tandemly arranged Ig- and mucin-binding protein (MucBP) modules. The binding of full-length MUB was confined to mucus via multiple interactions involving terminal sialylated mucin glycans. While individual MUB domains showed structural similarity to fimbrial proteins from Gram-positive pathogens, the particular organization of MUB provides a structural explanation for the mechanisms in which lactobacilli have adapted to their host niche by maximizing interactions with the mucus receptors, potentiating the retention of bacteria within the mucus layer. Together, this study reveals functional and structural features which may affect tropism of microbes across mucus and along the GI tract, providing unique insights into the mechanisms adopted by commensals and probiotics to adapt to the mucosal environment.

PubMed: 24373178
DOI: 10.1111/1462-2920.12377

実験手法

X-RAY DIFFRACTION (2.6 Å)