4MSS

Crystal structure of Burkholderia cenocepacia family 3 glycoside hydrolase (NagZ) bound to (3S,4R,5R,6S)-3-acetamido-4,5,6-trihydroxyazepane

Summary for 4MSS

• Entry DOI: 10.2210/pdb4mss/pdb
• Related: 4G6C 4GNV
• Descriptor: Beta-hexosaminidase 1, N-[(3S,4R,5R,6S)-4,5,6-trihydroxyazepan-3-yl]acetamide, GLYCEROL, ... (4 entities in total)
• Functional Keywords: tim barrel, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Burkholderia cenocepacia
• Total number of polymer chains: 2
• Total formula weight: 76918.26

Authors

Vadlamani, G.,Mark, B.L. (deposition date: 2013-09-18, release date: 2013-10-30, Last modification date: 2023-09-20)

Primary citation

Mondon, M.,Hur, S.,Vadlamani, G.,Rodrigues, P.,Tsybina, P.,Oliver, A.,Mark, B.L.,Vocadlo, D.J.,Bleriot, Y.
Selective trihydroxyazepane NagZ inhibitors increase sensitivity of Pseudomonas aeruginosa to beta-lactams.
Chem.Commun.(Camb.), 49:10983-10985, 2013

PubMed Abstract: AmpC β-lactamase confers resistance to β-lactam antibiotics in many Gram negative bacteria. Inducible expression of AmpC requires an N-acetylglucosaminidase termed NagZ. Here we describe the synthesis and characterization of hydroxyazepane inhibitors of NagZ. We find that these inhibitors enhance the susceptibility of clinically relevant Pseudomonas aeruginosa to β-lactams.

PubMed: 24136176
DOI: 10.1039/c3cc46646a

Experimental method

X-RAY DIFFRACTION (1.8 Å)