4ML7

Crystal structure of Brucella abortus PliC in complex with human lysozyme

4ML7 の概要

• エントリーDOI: 10.2210/pdb4ml7/pdb
• 分子名称: Lysozyme C, Humanlysozyme (3 entities in total)
• 機能のキーワード: inhibitor, lysozyme, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P61626
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 50735.33

構造登録者

Ha, N.C.,Um, S.H.,Kim, J.S. (登録日: 2013-09-06, 公開日: 2014-07-23, 最終更新日: 2024-11-06)

主引用文献

Um, S.H.,Kim, J.S.,Kim, K.,Kim, N.,Cho, H.S.,Ha, N.C.
Structural Basis for the Inhibition of Human Lysozyme by PliC from Brucella abortus
Biochemistry, 52:9385-9393, 2013

PubMed Abstract: Lysozymes are the first line of defense for a diverse range of organisms that catalyze the degradation of bacterial peptidoglycan. Gram-negative bacteria produce proteinaceous lysozyme inhibitors to protect themselves from the action of lysozymes. To date, MliC or PliC (membrane-bound or periplasmic inhibitor of c-type lysozyme, respectively) has been found in various Gram-negative bacteria. Here, we report the crystal structures of Brucella abortus PliC and its complex with human c-type lysozyme. The complex structure demonstrates that the invariant loop of MliC/PliC plays a crucial role in the inhibition of lysozyme via its insertion into the active site cleft of the lysozyme, as previously observed in the complex structure of Pseudomonas aeruginosa MliC and chicken c-type lysozyme. We identified a new binding interface between a loop adjacent to the active site of human lysozyme and a loop carrying Glu112 of B. abortus PliC, the structure of which was disordered in P. aeruginosa MliC. Because MliC/PliC family members have been implicated as putative colonization or virulence factors, the structures and mechanism of action of MliC/PliC will be relevant to the control of bacterial growth in animal hosts.

PubMed: 24308818
DOI: 10.1021/bi401241c

実験手法

X-RAY DIFFRACTION (1.8 Å)