4MJA

Synechocystis sp. PCC 6803 glutaredoxin A-A75I

4MJA の概要

• エントリーDOI: 10.2210/pdb4mja/pdb
• 関連するPDBエントリー: 3qmx 4MJB 4MJC 4MJE
• 分子名称: Probable glutaredoxin ssr2061 (2 entities in total)
• 機能のキーワード: glutaredoxin, oxidation/reduction, thioredoxin fold, electron transport
• 由来する生物種: Synechocystis sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11063.57

構造登録者

Sutton, R.B.,Knaff, D.B. (登録日: 2013-09-03, 公開日: 2013-12-04, 最終更新日: 2023-09-20)

主引用文献

Knaff, D.B.,Sutton, R.B.
Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins.
Front Plant Sci, 4:461-461, 2013

PubMed Abstract: Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.

PubMed: 24298277
DOI: 10.3389/fpls.2013.00461

実験手法

X-RAY DIFFRACTION (2 Å)