4MI7

Crystal Structure of Salmonella Effector Protein GtgE

4MI7 の概要

• エントリーDOI: 10.2210/pdb4mi7/pdb
• 分子名称: Bacteriophage encoded virulence factor, SULFATE ION (3 entities in total)
• 機能のキーワード: bacterial protein, toxin, hydrolase
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16041.86

構造登録者

Kohler, A.C.,Stebbins, C.E. (登録日: 2013-08-30, 公開日: 2014-02-12, 最終更新日: 2024-02-28)

主引用文献

Kohler, A.C.,Spano, S.,Galan, J.E.,Stebbins, C.E.
Structural and enzymatic characterization of a host-specificity determinant from Salmonella.
Acta Crystallogr.,Sect.D, 70:384-391, 2014

PubMed Abstract: GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 Å resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections.

PubMed: 24531472
DOI: 10.1107/S1399004713028393

実験手法

X-RAY DIFFRACTION (1.65 Å)