4MH8

The crystal structure of the monomeric reverse transcriptase from moloney murine leukemia virus

「1RW3」から置き換えられました

4MH8 の概要

• エントリーDOI: 10.2210/pdb4mh8/pdb
• 関連するPDBエントリー: 1rw3
• 分子名称: Reverse transcriptase/ribonuclease H p80 (1 entity in total)
• 機能のキーワード: rna and dna dependent dna polymerase, reverse transcriptase, transferase, replication, hydrolase
• 由来する生物種: Moloney murine leukemia virus (MoMLV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72573.55

構造登録者

Das, D.,Georgiadis, M.M. (登録日: 2013-08-29, 公開日: 2013-10-16, 最終更新日: 2023-09-20)

主引用文献

Das, D.,Georgiadis, M.M.
The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus.
Structure, 12:819-829, 2004

PubMed Abstract: Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.

PubMed: 15130474
DOI: 10.1016/j.str.2004.02.032

実験手法

X-RAY DIFFRACTION (3 Å)