4MB0

Crystal structure of TON1374

4MB0 の概要

• エントリーDOI: 10.2210/pdb4mb0/pdb
• 関連するPDBエントリー: 4MB2
• 分子名称: phosphopantothenate synthetase, ACETATE ION (3 entities in total)
• 機能のキーワード: ligase
• 由来する生物種: Thermococcus onnurineus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 118989.04

構造登録者

Kim, M.-K.,An, Y.J.,Cha, S.-S. (登録日: 2013-08-19, 公開日: 2014-08-06, 最終更新日: 2024-03-20)

主引用文献

Kim, M.-K.,An, Y.J.,Cha, S.-S.
The crystal structure of a novel phosphopantothenate synthetase from the hyperthermophilic archaea, Thermococcus onnurineus NA1
Biochem.Biophys.Res.Commun., 439:533-538, 2013

PubMed Abstract: Pantothenate is the essential precursor of coenzyme A (CoA), a fundamental cofactor in all aspects of metabolism. In bacteria and eukaryotes, pantothenate synthetase (PS) catalyzes the last step in the pantothenate biosynthetic pathway, and pantothenate kinase (PanK) phosphorylates pantothenate for its entry into the CoA biosynthetic pathway. However, genes encoding PS and PanK have not been identified in archaeal genomes. Recently, a comparative genomic analysis and the identification and characterization of two novel archaea-specific enzymes show that archaeal pantoate kinase (PoK) and phosphopantothenate synthetase (PPS) represent counterparts to the PS/PanK pathway in bacteria and eukaryotes. The TON1374 protein from Thermococcus onnurineus NA1 is a PPS, that shares 54% sequence identity with the first reported archaeal PPS candidate, MM2281, from Methanosarcina mazei and 91% sequence identity with TK1686, the PPS from Thermococcus kodakarensis. Here, we report the apo and ATP-complex structures of TON1374 and discuss the substrate-binding mode and reaction mechanism.

PubMed: 24021277
DOI: 10.1016/j.bbrc.2013.09.008

実験手法

X-RAY DIFFRACTION (1.96 Å)