4M6F

Dimer of the G-Segment Invertase bound to a DNA substrate

4M6F の概要

• エントリーDOI: 10.2210/pdb4m6f/pdb
• 関連するPDBエントリー: 1GDT 3PKZ 3UJ3
• 分子名称: DNA-invertase, gix site analog (3 entities in total)
• 機能のキーワード: helix-turn-helix, catalytic domain, dna binding domain, recombinase, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Enterobacteria phage Mu; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 32436.25

構造登録者

Ritacco, C.J.,Wang, J.,Steitz, T.A. (登録日: 2013-08-09, 公開日: 2014-03-12, 最終更新日: 2024-02-28)

主引用文献

Ritacco, C.J.,Steitz, T.A.,Wang, J.
Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex.
Acta Crystallogr.,Sect.D, 70:685-693, 2014

PubMed Abstract: Crystals of the G-segment invertase in complex with a 37-base-pair asymmetric DNA duplex substrate had an unusually high solvent content of 88% and diffracted to a maximal resolution of about 5.0 Å. These crystals exhibited a high degree of non-isomorphism and anisotropy, which presented a serious challenge for structure determination by isomorphous replacement. Here, a procedure of cross-crystal averaging is described that uses large non-isomorphous crystallographic data with a priori information of an approximate molecular boundary as determined from a minimal amount of experimental phase information. Using this procedure, high-quality experimental phases were obtained that have enabled it to be shown that the conformation of the bound substrate DNA duplex significantly differs from those of substrates bound in other serine recombinase-DNA complexes.

PubMed: 24598738
DOI: 10.1107/S1399004713032392

実験手法

X-RAY DIFFRACTION (4.99 Å)