4M45

RB69 DNA polymerase ternary complex with dG/dT at position n-5 of primer/template duplex

4M45 の概要

• エントリーDOI: 10.2210/pdb4m45/pdb
• 関連するPDBエントリー: 4M3R 4M3T 4M3U 4M3W 4M3X 4M3Y 4M3Z 4M41 4M42
• 分子名称: DNA polymerase, DNA template, DNA primer, ... (6 entities in total)
• 機能のキーワード: rb69 pol, quadruple, dg/dt, n-5, rb69, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Enterobacteria phage RB69; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 114078.24

構造登録者

Xia, S.,Konigsberg, W.H. (登録日: 2013-08-06, 公開日: 2014-02-12, 最終更新日: 2024-02-28)

主引用文献

Xia, S.,Konigsberg, W.H.
Mispairs with Watson-Crick base-pair geometry observed in ternary complexes of an RB69 DNA polymerase variant.
Protein Sci., 23:508-513, 2014

PubMed Abstract: Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson-Crick geometry in the presence of Mn(2+) indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base-pair could be affected by its microenvironment, we determined 10 structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n-1 to n-5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson-Crick geometry, have been observed, strongly suggesting that the local environment of base-pairs plays an important role in their tautomeric or ionization states.

PubMed: 24458997
DOI: 10.1002/pro.2434

実験手法

X-RAY DIFFRACTION (1.89 Å)