4M2O

Crystal structure of a non-myristoylated C39A recoverin mutant with one calcium ion bound to EF-hand 3

4M2O の概要

• エントリーDOI: 10.2210/pdb4m2o/pdb
• 関連するPDBエントリー: 1OMR 4M2P 4M2Q 4MLW
• 分子名称: Recoverin, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium binding protein, ef hand, neuronal calcium sensing (ncs) family protein, inhibits rhodopsin kinase, rhodopsin kinase, retina, metal binding protein
• 由来する生物種: Bos taurus (cow)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22613.41

構造登録者

Prem Kumar, R.,Ranaghan, M.J.,Oprian, D.D. (登録日: 2013-08-05, 公開日: 2013-11-13, 最終更新日: 2023-09-20)

主引用文献

Ranaghan, M.J.,Kumar, R.P.,Chakrabarti, K.S.,Buosi, V.,Kern, D.,Oprian, D.D.
A Highly Conserved Cysteine of Neuronal Calcium-sensing Proteins Controls Cooperative Binding of Ca2+ to Recoverin.
J.Biol.Chem., 288:36160-36167, 2013

PubMed Abstract: Recoverin, a 23-kDa Ca(2+)-binding protein of the neuronal calcium sensing (NCS) family, inhibits rhodopsin kinase, a Ser/Thr kinase responsible for termination of photoactivated rhodopsin in rod photoreceptor cells. Recoverin has two functional EF hands and a myristoylated N terminus. The myristoyl chain imparts cooperativity to the Ca(2+)-binding sites through an allosteric mechanism involving a conformational equilibrium between R and T states of the protein. Ca(2+) binds preferentially to the R state; the myristoyl chain binds preferentially to the T state. In the absence of myristoylation, the R state predominates, and consequently, binding of Ca(2+) to the non-myristoylated protein is not cooperative. We show here that a mutation, C39A, of a highly conserved Cys residue among NCS proteins, increases the apparent cooperativity for binding of Ca(2+) to non-myristoylated recoverin. The binding data can be explained by an effect on the T/R equilibrium to favor the T state without affecting the intrinsic binding constants for the two Ca(2+) sites.

PubMed: 24189072
DOI: 10.1074/jbc.M113.524355

実験手法

X-RAY DIFFRACTION (1.5 Å)