4LWB

Structure of Bacillus subtilis nitric oxide synthase in complex with 6-((((3R,5S)-5-(((6-amino-4-methylpyridin-2-yl)methoxy)methyl)pyrrolidin-3-yl)oxy)methyl)-4-methylpyridin-2-amine

Summary for 4LWB

• Entry DOI: 10.2210/pdb4lwb/pdb
• Related: 4LUW 4LUX 4LWA
• Descriptor: Nitric oxide synthase oxygenase, PROTOPORPHYRIN IX CONTAINING FE, 6-({[(3R,5S)-5-{[(6-amino-4-methylpyridin-2-yl)methoxy]methyl}pyrrolidin-3-yl]oxy}methyl)-4-methylpyridin-2-amine, ... (7 entities in total)
• Functional Keywords: nitric oxide synthase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• Biological source: Bacillus subtilis subsp. subtilis
• Total number of polymer chains: 1
• Total formula weight: 43314.00

Authors

Holden, J.K.,Li, H.,Poulos, T.L. (deposition date: 2013-07-26, release date: 2013-10-23, Last modification date: 2023-09-20)

Primary citation

Holden, J.K.,Li, H.,Jing, Q.,Kang, S.,Richo, J.,Silverman, R.B.,Poulos, T.L.
Structural and biological studies on bacterial nitric oxide synthase inhibitors.
Proc.Natl.Acad.Sci.USA, 110:18127-18131, 2013

PubMed Abstract: Nitric oxide (NO) produced by bacterial NOS functions as a cytoprotective agent against oxidative stress in Staphylococcus aureus, Bacillus anthracis, and Bacillus subtilis. The screening of several NOS-selective inhibitors uncovered two inhibitors with potential antimicrobial properties. These two compounds impede the growth of B. subtilis under oxidative stress, and crystal structures show that each compound exhibits a unique binding mode. Both compounds serve as excellent leads for the future development of antimicrobials against bacterial NOS-containing bacteria.

PubMed: 24145412
DOI: 10.1073/pnas.1314080110

Experimental method

X-RAY DIFFRACTION (2.15 Å)