4LU9

Crystal structure of E.coli SbcD at 2.5 angstrom resolution

4LU9 の概要

• エントリーDOI: 10.2210/pdb4lu9/pdb
• 関連するPDBエントリー: 4LTY
• 分子名称: Exonuclease subunit SbcD, GLYCEROL (3 entities in total)
• 機能のキーワード: meiotic recombination 11 homolog, double-strand break repair protein, nuclease, endonuclease, exonuclease, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 158238.81

構造登録者

Liu, S.,Tian, L.F.,Yan, X.X.,Liang, D.C. (登録日: 2013-07-25, 公開日: 2014-08-27, 最終更新日: 2024-03-20)

主引用文献

Liu, S.,Tian, L.F.,Liu, Y.P.,An, X.M.,Tang, Q.,Yan, X.X.,Liang, D.C.
Structural basis for DNA recognition and nuclease processing by the Mre11 homologue SbcD in double-strand breaks repair.
Acta Crystallogr.,Sect.D, 70:299-309, 2014

PubMed Abstract: The Mre11 complex comprising meiotic recombination 11 (Mre11), Rad50 and Nijmegen breakage syndrome 1 (Nbs1) plays multiple important roles in the sensing, processing and repair of DNA double-strand breaks (DSBs). Here, crystal structures of the Escherichia coli Mre11 homologue SbcD and its Mn2+ complex are reported. Dimerization of SbcD depends on a four-helix bundle consisting of helices α2, α3, α2' and α3' of the two monomers, and the irregular and bent conformation of helices α3 and α3' in the SbcD dimer results in a dimeric arrangement that differs from those of previously reported Mre11 dimers. This finding indicates a distinct selectivity in DNA substrate recognition. The biochemical data combined with the crystal structures revealed that the SbcD monomer exhibits single-stranded DNA (ssDNA) endonuclease activity and double-stranded DNA (dsDNA) exonuclease activity on the addition of a high concentration of Mn2+. For the first time, atomic force microscopy analysis has been used to demonstrate that the SbcD monomer also possesses Mn2+-dependent dsDNA endonuclease activity. Loop β7-α6 of SbcD is likely to be a molecular switch and plays an important role in the regulation of substrate binding, catalytic reaction and state transitions. Based on structural and mutational analyses, a novel ssDNA-binding model of SbcD is proposed, providing insight into the catalytic mechanism of DSBs repair by the Mre11 complex.

PubMed: 24531464
DOI: 10.1107/S139900471302693X

実験手法

X-RAY DIFFRACTION (2.5 Å)